Journal: The Journal of comparative neurology

Article Title: Absence of axonal sprouting following unilateral lesion in 125-day-old rat supraoptic nucleus may be due to age-dependent decrease in protein levels of ciliary neurotrophic factor receptor alpha

doi: 10.1002/cne.24675

Figure Lengend Snippet: CNTFRα protein levels decreased in the uninjured SON with age. Western blot analysis revealed a significant decrease in CNTFRα protein levels in the control SON from 35- to 125-days of age (a), but no change in CNTFRα protein levels in the maturing NL (c; p=0.2978). Analysis revealed no change in protein levels of CNTF in the maturing SON (b; p=0.07) or maturing NL (c; p=0.16), LIFRß in the maturing SON (b; p=0.10) or maturing NL (c; p=0.27), or gp130 in the maturing SON (b; p=0.28) or maturing NL (c; p=0.15). Each shape on the graph indicates an individual data point with the lines representing the mean and SD. Each data point represents isolated SON pooled from six rats with all experiments repeated in triplicate. *p<0.05, **p<0.01

Article Snippet: Antibody characterization Quantification of CNTF protein in the rat SON was achieved using rabbit anti-CNTF antibody (1:5000; #AAR21; Bio-Rad/AbD Serotec, Raleigh, NC; RRID:AB_2276485).

Techniques: Western Blot, Control, Isolation

Journal: The Journal of comparative neurology

Article Title: Absence of axonal sprouting following unilateral lesion in 125-day-old rat supraoptic nucleus may be due to age-dependent decrease in protein levels of ciliary neurotrophic factor receptor alpha

doi: 10.1002/cne.24675

Figure Lengend Snippet: Unilateral lesion results in increased levels of CNTF and CNTF receptor complex proteins in the 125-day-rat SON. Western blot analysis demonstrated a significant increase in CNTF (a; p=0.014), CNTFRα (b; p=0.0013), LIFRß (c; p=0.04), and gp130 (d; p<0.0001) protein levels in the injured (lesion) SON at 10 dpl. However, there was no significant difference in the protein levels in the SON contralateral to injury compared to age-matched, uninjured, control for CNTF (a; p=0.23), CNTFRα (b; p=0.075), LIFRß (c; p=0.07), and gp130 (d; p=0.36). Each shape on the graph indicates an individual data point with the lines representing the mean and SD. Each data point represents isolated SON pooled from six rats with all experiments repeated in triplicate. *p<0.05, **p<0.01, ***p<0.0001.

Article Snippet: Antibody characterization Quantification of CNTF protein in the rat SON was achieved using rabbit anti-CNTF antibody (1:5000; #AAR21; Bio-Rad/AbD Serotec, Raleigh, NC; RRID:AB_2276485).

Techniques: Western Blot, Control, Isolation

Journal: The Journal of comparative neurology

Article Title: Absence of axonal sprouting following unilateral lesion in 125-day-old rat supraoptic nucleus may be due to age-dependent decrease in protein levels of ciliary neurotrophic factor receptor alpha

doi: 10.1002/cne.24675

Figure Lengend Snippet: Protein levels of CNTF and CNTF receptor complex increase in the 35-day rat NL following unilateral lesion. Western blot analysis demonstrated a significant increase in CNTF (a; p=0.002), CNTFRα (b; p<0.0001), LIFRß (c; p=0.0003), and gp130 (d; p=0.018) protein levels in the lesion NL compared to age-matched control. Each shape on the graph indicates an individual data point with the lines representing the mean and SD. Each data point represents isolated SON pooled from six rats with all experiments repeated in triplicate. *p<0.05, **p<0.01, ***p<0.0001.

Article Snippet: Antibody characterization Quantification of CNTF protein in the rat SON was achieved using rabbit anti-CNTF antibody (1:5000; #AAR21; Bio-Rad/AbD Serotec, Raleigh, NC; RRID:AB_2276485).

Techniques: Western Blot, Control, Isolation

Journal: Stem cell research & therapy

Article Title: Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function.

doi: 10.1186/s13287-018-0884-3

Figure Lengend Snippet: Fig. 3 Relative messenger RNA (mRNA) expression and neurotrophin secretion levels of all groups. a The relative expression level of nerve growth factor receptor (NGFR) (p75) mRNA was not significantly different among sustaining dASCs 7d (5.55 ± 0.29), intermittent dASCs (5.11 ± 0.23), and SCs (5.54 ± 0.34), which were significantly higher (p < 0.01) than the other three groups. b The relative expression level of P0 mRNA was highest in SCs (14.11 ± 0.88). There were no significant differences between intermittent dASCs (8.46 ± 0.34) and sustaining dASCs 7d (7.10 ± 0.54). However, intermittent dASCs showed significantly higher P0 mRNA expression (p < 0.05) in comparison with sustaining dASCs 10d (6.00 ± 0.12) and 4d groups (2.66 ± 0.19). c The relative expression level of glial fibrillary acidic protein (GFAP) mRNA was not significantly different between intermittent dASCs (4.62 ± 0.21) and the three sustaining dASCs groups, all of which showed significant upregulation (p < 0.01) in comparison with the undifferentiated adipose-derived stem cells (uASCs). d The levels of nerve growth factor (NGF) secreted by intermittent dASCs (99.37 ± 5.00 pg/ml) and sustaining dASCs 7d (95.20 ± 4.34 pg/ml) were significantly higher (p < 0.01) than those of uASCs (31.18 ± 3.09 pg/ml), and sustaining dASCs 4d (54.69 ± 2.20 pg/ml) and 10d (45.90 ± 2.27 pg/ml), but lower (p < 0.01) than that of SCs (112.46 ± 4.55 pg/ml). e,f Brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) secretion levels showed similar tendencies. The concentrations of BDNF and GDNF secreted by intermittent dASCs were 483.01 ± 10.08 and 205.66 ± 6.01 pg/ml, respectively, which were higher (p < 0.01) than in the other groups, including SCs. g The concentrations tendency of ciliary neurotropic factor (CNTF) and NGF were similar. Data are expressed as means ± SEM. *p < 0.05, **p < 0.01, one-way ANOVA with Tukey’s post-test or Dunnett’s T3 post-test. dASC, differentiated adipose-derived stem cell; n.s., no significant difference, SC, Schwann cell

Article Snippet: The concentrations of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), and ciliary neurotrophic factor (CNTF) in the supernatant of each group were examined by ELISA using Rat NGF/NGF beta ELISA Kit (EK0471; Boster), Rat BDNF ELISA Kit (EK0308; Boster), Rat GDNF ELISA Kit (EK0363; Boster), and Rat CNTF ELISA Kit (EK0324; Boster), respectively.

Techniques: Expressing, Comparison, Derivative Assay